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Sigma Aldrich Fine Chemicals Biosciences fastStart TaqManR Probe Ma
Supplier: Sigma Aldrich Fine Chemicals Biosciences 4673417001
Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore there is no elongation during the period when primers can nonspecifically bind. The fastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
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