Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.fastStart(TM) Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX without modification or adjustments to the specific instrument or protocol. This ready-to-use 2x concentrated master mix contains all reagents (except primers probe and template) needed for running quantitative real-time DNA-detection assays including qPCR and two-step qRT-PCR in the hydrolysis probe detection format. fastStart(TM) Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler(R) Instruments.