Sigma Aldrich Fine Chemicals Biosciences FastStartTM High Fidelity

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 3553361001

The FastStart(TM) High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified. This protein mediates proofreading activity but carries no polymerase activity. Both proteins are inactive below 75 C and are activated by heating to 95 C for two minutes.Hot start protocols have improved specificity sensitivity and PCR yield. Heat-labile blocking groups on amino acid residues of FastStart(TM) Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. FastStart(TM) Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at 95C. The FastStart(TM) High Fidelity PCR System is the product of choice for multiplex PCR. FastStart(TM) High Fidelity Enzyme Blend (5 U/mul) in storage bufferFastStart(TM) High Fidelity Reaction Buffer 10x concentrated with 18 mM MgCl2FastStart(TM) High

Catalog No. 50-290-7517