Sigma Aldrich Fine Chemicals Biosciences fastStartTM High fidelity

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 4738292001

The enzyme dNTPack comprises the fastStart High fidelity PCR System and a ready-to-use SOLUTIONon of PCR grade nucleotides. The fastStart High fidelity PCR System is a blend of fastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified. This protein mediates proofreading activity but carries no polymerase activity. Both proteins are inactive below 75C and are activated by heating to 95C for two minutes. The fastStart(R) High fidelity PCR System is the ideal tool for all amplification experiments where a combination of high specificity sensitivity accuracy and yield is needed. Hot start protocols have improved specificity sensitivity and PCR yield. Heat-labile blocking groups on amino acid residues of fastStart Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically.

Catalog No. 50-290-4897