AdipoGen Fc (human):FGL1 (mouse) (rec.) (3 x 10 µg)

Supplier:  AdipoGen AG40B01853010

Recombinant Protein. Mouse FGL1 (aa 23-314) is fused at the N-terminus to the Fc portion of human IgG1 and at the C-terminus to a His-Tag. Should activate mouse LAG-3 (activity test not yet performed). Source/Host: HEK 293 cells. Endotoxin: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. FGL1 has been shown to be upregulated in human cancers and FGL1 is a major functional ligand of LAG-3. FGL1 interacts with LAG-3 in an MHC-II-independent manner and this interaction involves the FGL1 fibrinogen-like domain and the LAG-3 D1-D2 domain. FGL1-LAG-3 interaction blockade promotes tumor immunity by stimulating T cell expansion and activation. FGL1 forms two disulfide-linked homodimers and also higher molecular weight homooligomers that bind to LAG-3 much better than the dimeric forms. This binding to LAG-3 inhibits T cell responses. The recombinant fusion protein Fc (human):FGL1 (human) (FGL1-Ig) has been shown to function as an efficient ligand of LAG-3.

Catalog No. 50-205-3285