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ACROBiosystems Recombinant Protein;Biotinylated Human Fc gamma RI / CD64 Protein, His Tag, Avi Tag (Avitag™) (SPR verified);HEK293;200UG;FCA,FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI,FCGR-1,IGFR-1,Fcr,Fcgr,Fc receptor,Fc gamma receptor
Supplier: ACROBiosystems FCAH82E8200UG
Recombinant Protein;Biotinylated Human Fc gamma RI / CD64 Protein, His Tag, Avi Tag (Avitag™) (SPR verified);200UG;Biotinylated Human CD64, His Tag, Avi Tag (SPR verified) (FCA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # P12314-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 34.3 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Yervoy Ipilumimab at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His Tag, Avi Tag (SPR verified) (Cat. No. FCA-H82E8) with a linear range of 0.2-3 ng/mL (QC tested).;Biotinylated Human CD64, His Tag, Avi Tag (SPR verified) (Cat. No. FCA-H82E8) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 1.02 nM as determined in a SPR assay (Biacore T200) (QC tested).
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