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ACROBiosystems ACROBiosystems Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
Supplier: ACROBiosystems CDBM52H750UG
Recombinant Protein;50UG;Mouse CD32b, His Tag (SPR & BLI verified) (CDB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657.1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) on NTA-Series S sensor chip, can bind OKT3 with an affinity constant of 0.86 μM as determined in a SPR assay (Biacore T200) (QC tested).;Immobilized OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-M52H7) with an affinity constant of 0.55 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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