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ACROBiosystems Recombinant Protein;Biotinylated Human Fc gamma RIIB / CD32b Protein, His Tag, Avi Tag (Avitag™) (SPR verified);HEK293;25UG;CDB,FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32,FCG-2,IGFR-2,Fcr,Fcgr,Fc receptor,Fc gamma receptor
Supplier: ACROBiosystems CDBH82E025UG
Recombinant Protein;Biotinylated Human Fc gamma RIIB / CD32b Protein, His Tag, Avi Tag (Avitag™) (SPR verified);25UG;Biotinylated Human CD32b, His Tag, Avi Tag (SPR verified) (CDB-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1).;This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 23.0 kDa. The protein migrates as 25 kDa and 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Biotinylated Human CD32b, His Tag, Avi Tag (Cat. No. CDB-H82E0) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 5 μM as determined in a SPR assay (Biacore T200) (QC tested). ;Immobilized Biotinylated Human CD32b, His Tag, Avi Tag (Cat. No. CDB-H82E0) on SA Chip, can bind Rituximab with an affinity constant of 4.22 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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