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ACROBiosystems ACROBiosystems Biotinylated Human Fc gamma RIIIA / CD16a (F176) Protein, Avitag™,His Tag (SPR & BLI verified)
Supplier: ACROBiosystems CDAH82E8200UG
Recombinant Protein;200UG;Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) (CDA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).;This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E8) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 1.72 μM as determined in a SPR assay (Biacore T200) (QC tested). ;Immobilized Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E8) on SA Chip can bind Rituximab with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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