Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Manufacturer: Thermo Scientific™ R0862
DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state .
At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (1). At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.
- Free of endo-, exodeoxyribonucleases, ribonucleases and phosphatases
Enzyme stabilization; Enzymatic reactions; Protein electrophoresis.
DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1M DTT solution, dissolve 1.55g of DTT powder in 10mL of deionized water (e.g., Water, nuclease-free).
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