Thermo Scientific™ GeneJET Gel Extraction and DNA Cleanup Micro Kit

Description

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer. The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 μg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup

Highlights
• Advanced column design—easy to open, load and handle
• Yields concentrated DNA - Elute in 6 to 10 μL volume
• High recovery - 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
• Fast—4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
• Efficient removal of reaction contaminants - including enzymes, proteins, primers dimers, dNTPs, and salts
• Flexible—does not require precise gel slice quantification weighting

Applications
The purified DNA can be used in common downstream applications:
• Sequencing
• Restriction digestion
• PCR
• qPCR
• Labeling
• Ligation
• Cloning
• In vitro transcription
• Blotting
• In situ hybridization

Includes
• Binding Buffer
• Extraction Buffer
• Prewash Buffer (conc.)
• Wash Buffer (conc.)
• Elution Buffer
• DNA Purification Micro Column & Collection Tube

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Specifications

• Column Type: Spin Column
• Format: Spin Column
• Isolation Technology: Silica Column
• Sample Type: DNA, Gel Samples
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: GeneJET
• Product Type: Gel Extraction and DNA Cleanup Kit
• Quantity: 250 Preps
• Target: DNA from Enzymatic Reactions (e.g. PCR)
• Test Time: 15 min.

For Research Use Only. Not for use in diagnostic procedures.