Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Catalog No.	Quantity
FERK0481	25 Preps
FERK0482	100 Preps

Catalog No. FERK0481 Supplier Thermo Scientific™ Supplier No. K0481

Description

Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines

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Specifications

• Concentration: 400 ng/μL
• Column Type: Spin Column
• Format: Spin Column
• Isolation Technology: Silica Column
• Scale: Midi
• Sample Type: Bacteria
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
• Product Line: GeneJET
• Product Type: Plasmid MidiPrep Kit
• Quantity: 25 Preps
• Target: Plasmid DNA
• Test Time: 60 min.

Frequently Asked Questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

How can I increase plasmid DNA yield during elution from GeneJET plasmid kits?

To increase the overall plasmid DNA yield by 20-30%, additional elution steps may be performed.

Which culture media are recommended for bacteria to be used with GeneJET Plasmid Kits? Can I use a rich growth medium?

The GeneJET Plasmid purification protocols are optimized for bacterial cultures grown in Luria-Bertani (LB) medium. The use of rich growth media is not recommended.

Which bacterial strains can be used for plasmid purification with GeneJET Plasmid KitS?

High quality plasmid DNA can be obtained from various E. coli strains including DH10B, DH5a, XL1-Blue, JM109, JM107, and TOP10.

For Research Use Only. Not for use in diagnostic procedures.