Thermo Scientific™ GeneJET RNA Cleanup and Concentration Micro Kit

Catalog No.	Quantity
FERK0842	250 Preps
FERK0841	50 Preps

Catalog No. FERK0842 Supplier Thermo Scientific™ Supplier No. K0842

Description

GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Thermo Scientific GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Highlights

• Advanced column design—easy to open, load and handle.
• Elute in 6 to 10 μL volume.
• 85 to 100% recovery of a high integrity RNA.
• Fast and simple—procedure takes less than 5 minutes.

Applications:

• The purified RNA can be used in common downstream applications
• RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis

Specifications

• Content And Storage: For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
• Format: Spin Column
• Isolation Technology: Spin Column
• Purification Time: 5 min.
• Sample Type: RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
• No. of Reactions: 250 Preps
• Product Line: GeneJET
• Quantity: 250 Preps
• Scale: Micro
• Target: Total RNA

Frequently Asked Questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

For Research Use Only. Not for use in diagnostic procedures.