Thermo Scientific™ Genomic DNA Purification Kit

Description

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

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Specifications

• Content And Storage: • 40 mL Lysis Solution; room temperature
  • 8 mL Precipitation Solution (concentrated); room temperature
  • 10 mL NaCl Solution; room temperature
  
  
• Isolation Technology: Organic Extraction
• Sample Type: Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
• Elution Volume: 20 to 100 μL
• Final Product Type: gDNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 100 Preps
• Shipping Condition: Room Temperature
• Test Time: 25 min.
• Starting Material Amount: Bacteria: ≤20 mg
  Cells: ≤10⁶
  Plant: ≤100 mg
  Tissue: ≤30 mg
  Serum: ≤200 μL
  Whole Blood: ≤200 μL

For Research Use Only. Not for use in diagnostic procedures.