Thermo Scientific™ Lambda DNA/EcoRI plus HindIII Marker

Catalog No.	Quantity
FERSM0191	5 x 50 μg
FERSM0192	25 x 50 μg

Catalog No. FERSM0191 Supplier Thermo Scientific™ Supplier No. SM0191

Description

Perform sizing and approximate quantification of large double-stranded DNA fragments in agarose gels with this ladder that consists of 13 sharp bands.

Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.

Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reactio

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note:

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated™ may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min. and then cooling on ice for 3 min.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences.

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Specifications

• Gel Type: Agarose
• Ready to Load: No
• Gel Compatibility: Agarose Gels
• Quantity: 5 x 50 μg
• Product Type: DNA/ECORI Plus Hind III Marker

Frequently Asked Questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.