Thermo Scientific™ X-Gal Solution, ready-to-use

Description

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Formula: C₁₄H₁₅BrClNO₆ Molecular weight: 408.6

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Recommended for:

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.

Note:

Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

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Specifications

• Detection Method: Colorimetric
• Product Type: X-Gal Solution
• Quantity: 10 mL
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate, X-Gal

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.