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Thermo Scientific™ Silica Bead DNA Gel Extraction Kit
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Catalog No. FERK0513
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FERK0513 100 Preps
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Catalog No. FERK0513 Supplier Thermo Scientific™ Supplier No. K0513
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Description

Perform silica bead-based DNA extraction from agarose gels and reaction mixtures with this kit, which is flexible to scale up or down both reaction and elution volumes.

Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie , employing solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water.

This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling reaction and elution volumes up or down. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.

Highlights

  • Flexible—easy to scale up or down both reaction and elution volumes
  • Efficient—greater than 80% DNA recovery
  • Fast—purification takes only 15 to 20 minutes
  • Effective—suitable for DNA fragments as short as 100 bp
  • Safe—does not involve phenol extraction

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Specifications

Format Kit
Sample Type DNA, Gel Samples
For Use With (Application) Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Type Silica Bead DNA Gel Extraction Kit
Quantity 100 Preps
Target DNA from Enzymatic Reactions (e.g. PCR)
Test Time 15 to 20 min.

For Research Use Only. Not for use in diagnostic procedures.

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