Thermo Scientific™ X-Gluc

Description

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Recommended for:

• Detection of GUS expression in plant cells and tissues (1)
• Detection of infections caused by E.coli
• Detection of bacterial contamination in food and water samples

Note:

Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

Specifications

• Detection Method: Colorimetric
• Product Type: X-Gluc
• Quantity: 0.1 g
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Glucuronidase (GUS) Substrate

For Research Use Only. Not for use in diagnostic procedures.