Invitrogen™ Human FETUB/Fetuin B ELISA Kit

Catalog No.	Quantity
EHFETUBX10	10 x 96 Tests

Catalog No. EHFETUBX10 Supplier Invitrogen™ Supplier No. EHFETUBX10

Description

ELISA

The Human FETUB (Fetuin B) ELISA quantitates Hu FETUB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FETUB. Principle of the method The Human FETUB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.

Specifications

• Accession Number: Q9UGM5
• Assay Range: 4.39-50 ng/mL
• Assay Sensitivity: 4 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cell Structure/Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 16G2; 2310011O17Rik; AI255764; D17980; Fet; FETUB; fetuin B; fetuin beta; fetuin phosphoprotein; fetuin-B; Fetuin-like protein IRL685; Gugu; IRL685; Pp6316G2; 2310011O17Rik; AI255764; D17980; Fet; FETUB; fetuin B; fetuin beta; fetuin phosphoprotein; fetuin-B; Fetuin-like protein IRL685; Gugu; IRL685; Pp63; 16G2; Gugu; IRL685
• Gene ID (Entrez): 26998
• Gene Symbol: FETUB
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: 16G2, Fetuin-like protein IRL685, Gugu
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Fetuin B
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min