Human Fetuin-B (FETUB), also known as Fetuin-like protein IRL685, 16G2, and Gugu, is a liver-derived plasma protein essential for fertilization. It acts as a protease inhibitor that prevents premature zona pellucida hardening by inhibiting the protease activity of ASTL. This recombinant protein is expressed in human HEK293 cells and contains a C-terminal polyhistidine tag.
- Greater than 95% purity as determined by SDS-PAGE.
- Calculated molecular weight of 42.4 kDa, migrating at 53-58 kDa under reducing conditions due to glycosylation.
- Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0 with trehalose as a protectant.
- Endotoxin level less than 1.0 EU per μg by LAL or rFC method.
- Stable for 12 months at -20°C to -70°C in lyophilized state.