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Cayman Chemical FFAR4 (GPR120) Reporter Assay Kit, 1 ea
SDP

Catalog No. 502894056
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FFAR4/GPR120 is a G protein-coupled receptor for long chain fatty acids such as DHA and EPA ω-3 fatty acids.; Cayman’s FFAR4 (GPR120) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for the short isoform of FFAR4 (FFAR4S), an engineered G protein that directs Gαq activation signals to the Gαs pathway, and a cAMP response element-regulated SEAP reporter (FFAR4 reverse transfection strip plate).; Binding of agonists to FFAR4S initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium.; Aliquots of culture medium are collected 16-24 hours after stimulation and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.; The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.

Catalog No. 50-289-4056 Supplier Cayman Chemical Supplier No. 6012001 EA
May include imposed supplier surcharges.
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FFAR4/GPR120 is a G protein-coupled receptor for long chain fatty acids such as DHA and EPA ω-3 fatty acids.; Cayman’s FFAR4 (GPR120) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for the short isoform of FFAR4 (FFAR4S), an engineered G protein that directs Gαq activation signals to the Gαs pathway, and a cAMP response element-regulated SEAP reporter (FFAR4 reverse transfection strip plate).; Binding of agonists to FFAR4S initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium.; Aliquots of culture medium are collected 16-24 hours after stimulation and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.; The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.

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