Recombinant rat fibroblast growth factor 23 supplied lyophilized; expressed in Escherichia coli and provided as a His-tagged fragment corresponding to amino acids Y25-V251. The protein is intended for in vitro biochemical assays, binding studies, and assay development and includes guidance for reconstitution and storage.
- Recombinant rat FGF-23 (Y25-V251) with His tag.
- Expressed in Escherichia coli and purified with low endotoxin.
- Lyophilized from 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
- Endotoxin level <1 EU/μg as determined by LAL method.
- Approximate molecular weight 25-30 kDa by SDS-PAGE under reducing conditions.
- Do not reconstitute below 100 μg/mL; after reconstitution stable at 4°C for 1 week or at -20°C longer with carrier protein.
- Recommended to aliquot and store at -20°C or -80°C for extended storage.
- Suitable for biochemical assays, binding studies, and assay development.