Invitrogen™ Human FGF1 (FGF alpha) ELISA Kit

Catalog No.	Quantity
EHFGF1X5	5 x 96 Tests
EHFGF1	96 Tests
EHFGF1X10	10 x 96 Tests

Catalog No. EHFGF1X5 Supplier Invitrogen™ Supplier No. EHFGF1X5

Description

ELISA

The Human Fibroblast Growth Factor 1 (Hu FGF1/FGFα) ELISA quantitates Hu FGF1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF1. Principle of the method The Human FGF1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

Specifications

• Accession Number: P05230
• Assay Range: 12-4000 pg/mL
• Assay Sensitivity: 12 pg/mL
• Calibration Range: 12 pg/mL to 4000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Growth Factors & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human BLC, BDNF, bFGF, BMP-4, BMP-5, BMP-7, b-NGF, Eotaxin-1, Eotaxin-2, FGF-4, FGF-7, G-CSF, GM-CSF, I-309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, sTNFRI, TIMP-1, TIMP-2, TNF alpha, TNF beta, VEGF, VEGFR2, VEGFR3.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: acidic eye-derived fibroblast growth factor; acidic eye-derived growth factor II; Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; Dffrx; ECGF; ECGFA; ECGFB; ECGF-beta; EDGF; EDGF II; Endothelial cell growth factor; Endothelial cell growth factor alpha; Endothelial cell growth factor beta; endothelial cell growth factor beta and alpha chains; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; endothelial growth factor; Fam; FGF; FGF1; Fgf-1; FGF1 protein; Fgfa; FGF-alpha; Fibroblast growth factor; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); Fibroblast growth factor 1 (heparin binding); fibroblast growth factor, acidic; GLIO703; HBGF1; HBGF-1; heparin-binding growth factor; Heparin-binding growth factor 1; H-FGF-a; M-FGF-a; Prostatropin; unnamed protein productacidic eye-derived fibroblast growth factor; acidic eye-derived growth factor II; Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; Dffrx; ECGF; ECGFA; ECGFB; ECGF-beta; EDGF; EDGF II; Endothelial cell growth factor; Endothelial cell growth factor alpha; Endothelial cell growth factor beta; endothelial cell growth factor beta and alpha chains; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; endothelial growth factor; Fam; FGF; FGF1; Fgf-1; FGF1 protein; Fgfa; FGF-alpha; Fibroblast growth factor; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); Fibroblast growth factor 1 (heparin binding); fibroblast growth factor, acidic; GLIO703; HBGF1; HBGF-1; heparin-binding growth factor; Heparin-binding growth factor 1; H-FGF-a; M-FGF-a; Prostatropin; unnamed protein product; AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1
• Gene ID (Entrez): 2246
• Gene Symbol: FGF1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Angiogenesis
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1
• Target: FGF1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min