Invitrogen™ Human FGFR3 [G697C], GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
PV6186	1 mg
PV6185	100 μg
PV6184	10 μg

Catalog No. PV6186 Supplier Invitrogen™ Supplier No. PV6186

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human FGFR3, C-terminal fragment, amino acids R397-T806 (as in NCBI/Protein entry NP_000133.1) with a G697C mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM HEPES with 5mM DTT, 20% glycerol, 15mM glutathione, 100mM NaCl and no preservative; pH 7.5
• Molecular Weight (g/mol): 74 kDa
• Name: Human FGFR3 [G697C], GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: ACH, CD333, CEK2, FGFR-3, HSFGFR3EX, JTK4
• Common Name: FGFR3 (G697C Mutant)
• Protein Length: aa 397-806
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 397-806
• Content And Storage: Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Receptor Tyrosine Kinase
• Expression System: Insect cells
• Protein Form: Recombinant
• Form: Liquid
• Protein Subtype: FGFs (Fibroblast Growth Factors)