Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit

Description

Includes

Lysis solution (20mL); Wash solution (22mL); Binding solution (25mL); RNA elution solution (6mL); Spin columns (50); Collection tubes (50); Elution tubes (1.7mL) (50); Product insert (1)

Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples

• Fast and easy processing using rapid spin-column format
• High quality intact RNA can be used in various downstream applications
• Enrich for cytoplasmic or nuclear RNA depending on downstream application
• No genomic DNA contamination in the cytoplasmic fraction
• No phenol or chloroform extractions
• Isolate all sizes of RNA from each fraction, including all small RNA molecules (>200 nt)
• Kit provides sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and nuclear preparations

Applications:

Purification of Nuclear and Cytoplasmic RNA suitable for real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Specifications

• Format: Spin Column
• For Use With (Application): RT-PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension and expression array assays
• Includes: Lysis solution 20mL, wash solution 22mL, binding solution 25mL, RNA elution solution 6mL, spin columns 50, collection tubes 50, elution tubes 1.7mL 50, product insert 1 50μg Column Binding Capacity, 600μL Max. column lo
• Quantity: 75 Preps
• Yield: 35μg Grape leaves (50mg), 32μg plum leaves (50mg), 60μg tobacco leaves (50mg), 60μg tomato leaves (50mg)

Safety and Handling

SDS