Thermo Scientific™ Flag™ Magnetic Agarose Purification Bundle

Description

Includes

Pierce Anti-DYKDDDDK Magnetic Agarose (1 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Pierce IP Lysis Buffer (100 mL), Pierce IgG Elution Buffer, pH 2.8 (1 L)

Flag™ Magnetic Agarose Purification Bundle combines optimized reagents for success in purifying FLAG-tagged proteins. The bundle includes Pierce Anti-DYKDDDK Magnetic Agarose, Pierce IP Lysis buffer and Halt Protease Inhibitor Cocktail.

• Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as “FLAG™”, is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle
• Thermo Scientific Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate. IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer
• Thermo Scientific Halt Protease Inhibitor Cocktail with IP lysis buffer added before use, protects proteins from proteolytic degradation through inhibition of serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples

Specifications

• Quantity: 1 Bundle
• Includes: Pierce Anti-DYKDDDDK Magnetic Agarose (1 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Pierce IP Lysis Buffer (100 mL), Pierce IgG Elution Buffer, pH 2.8 (1 L)
• Content And Storage: See instructions for individual components
• Matrix: Magnetic Agarose