Invitrogen™ Human FLT3 [ITD], GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
PV6192	1 mg
PV6190	10 μg
PV6191	100 μg

Catalog No. PV6192 Supplier Invitrogen™ Supplier No. PV6192

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human FLT3, C-terminal fragment, amino acids R571-S993 (as in NCBI/Protein entry NP_004110.2), internal tandem duplication (oncological mutation: ITD) of aa 591-600 N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM HEPES with 20% glycerol, 5mM DTT, 100mM NaCl, 15mM glutathione and no preservative; pH 7.5
• Molecular Weight (g/mol): 78.6 kDa
• Name: Human FLT3 [ITD], GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CD135, EC 2.7.10.1, FLK2, STK1
• Common Name: FLT3 (ITD Mutant)
• Protein Length: aa 571-993
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 571-993
• Content And Storage: Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Receptor Tyrosine Kinase
• Expression System: Baculovirus
• Protein Form: Recombinant
• Form: Liquid
• Protein Subtype: FLT-3 Ligand