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Abcam Fluo-8 AM, green fluorescent calcium binding dye, 1 mg, solid, soluble in DMSO, excitation 490 nm, emission 520 nm, medium calcium affinity (Kd 390 nM)
Supplier: Abcam AB1427731MG
Fluo-8 AM is a cell-permeable, medium-affinity green fluorescent calcium-binding dye specifically designed for detecting intracellular calcium levels with high sensitivity and robustness. This dye displays a significant enhancement in fluorescence intensity when bound to Ca2+ (Kd = 390 nM) and offers a fluorescence signal that is 1.5 times brighter than Fluo-4 in cellular studies. With optimal excitation and emission wavelengths at 490 nm and 520 nm respectively, Fluo-8 AM is ideal for live imaging of cytosolic calcium in various functional assays in scientific research.
- Cell-permeable dye
- Medium calcium affinity (Kd = 390 nM)
- Fluorescent color: Green
- Excitation maximum: 490 nm
- Emission maximum: 520 nm
- Soluble in DMSO
- Physical form: Solid
- Storage temp: -20°C
- Stable up to 12 months
- 1.5x brighter than Fluo-4
- Cited in over 50 publications
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