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Apexbio Technology LLC Firefly Luciferase mRNA (ARCA 5mCTP ψUTP) 1mg (1mg/mL)
Supplier: Apexbio Technology LLC R10051.0
Firefly Luciferase mRNA (ARCA 5mCTP UTP) encodes firefly luciferase protein originally isolated from Photinus pyralis Firefly luciferase is a widely used bioluminescence reporter molecule for studies on gene regulation cell viability ATP levels and in vivo imaging In the presence of ATP and oxygen luciferase catalyzes the oxidation of D-Luciferin (Cat C3654) emitting chemiluminescence at 560 nm This product is a 5 -capped 3 -poly(A)-tailed mRNA modified with 5mCTP and UTP mimicking mature eukaryotic mRNA Capped with ARCA (anti-reverse cap analog Cat B8175) via co-transcription it forms a Cap 0 structure enhancing stability and translation efficiency [1 2] The 5mCTP (Cat B7967) and UTP (Pseudo-UTP Cat B7972) modifications reduce innate immune activation and increase mRNA stability The poly(A) tail boosts stability and translation initiation efficiency
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