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AdipoGen Fluo-3 AM
SDP

Supplier:  AdipoGen CDXF0033M001

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Chemical. CAS 121714-22-5. Formula C51H50Cl2N2O23. MW 1129.85. Synthetic. Ca2-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2. The green-fluorescent emission ~525 nm of Ca2-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein FITC. As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2.

Catalog No. 50-256-0927


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