Dojindo Molecular Technologies Inc Fluorescein Labeling Kit-NH2 (3 samples) is used to prepare fluorescein-labeled proteins, high recovery in 1 hour, all processes in a single filtration tube, 50-200 µg/sample, Ex=500 nm, Em=525 nm.

Supplier:  Dojindo Molecular Technologies Inc LK0110

Fluorescein Labeling Kit-NH2 is mainly used for the preparation of fluorescein-labeled proteins, such as IgG, for immunostaining and labeled cellular proteins for tracing. This kit contains all the reagents necessary for labeling. Amine-reactive fluorescein, a component of this kit, has succinimidyl groups (NHS) that react with the amino groups on proteins. Because this kit also includes a buffer exchange system, a sample containing amine base buffer can be labeled. Although membrane filtration sometimes causes IgG aggregation, the buffer system in this kit prevents aggregation during the concentration of IgG or fluorescein-labeled IgG solution. The excitation and emission wavelengths of the fluorescein-labeled IgG are 500 nm and 525 nm. The molecular weight of the protein to be labeled with this kit should be greater than 50,000. The amount of the protein is 50 ug-200 ug per sample. Dojindo offers various protein labeling reagents. (https://www.dojindo.com/labeling-chemistry/)

Catalog No. NC1960989