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Rockland Immunochemicals Fluorescent TrueBlot: Anti-Mouse Ig DyLight™ 800 - 18-4517-32
Supplier: Rockland Immunochemicals 18451732
Fluorescent Mouse TrueBlot Antibody DyLight™ 800 may also be used for detection in immunoassays that do not employ IP. Provided as a lyophilized powder. We recommend incubating the blots from minigels in sealed bags with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 40 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. 2. Immunoprecipitate should be completely reduced. 3. BSA or MB-070 Blocking Buffer for Fluorescent WB, at low concentrations, should be used as the blocking protein for the WB. DO NOT USE BLOTTO or MILK. Note: To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user.
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