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Rockland Immunochemicals Fluorescent TrueBlot: Anti-Rabbit IgG DyLight™ 680 - 18-4416-32
Supplier: Rockland Immunochemicals 18441632
Fluorescent Rabbit TrueBlot Antibody DyLight™ 680 may also be used for detection in immunoassays that do not employ IP. Pprovided as a lyophilized powder. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 40 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the IP. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For IP, anti-rat IgG beads, or anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. MB-070 Blocking Buffer for Fluorescent WB should be used as the blocking protein for the immunoblot. Specific conditions for each protein and antibody combination should be specifically optimized by the end user.
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