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Innovative Research Inc Fngs-Der Rbncls T1 Anml Fr Pur
Supplier: Innovative Research Inc IFGRNASET1AF100KU
Fungus-Derived Ribonuclease T1 Animal Free Purified 100ku - Fungus-Derived Ribonuclease T1 Animal Free Purified from Innovative Research has been prepared as a highly purified, microbial (non-mammalian) RNase prepared with non-animal components. This product is a non-mammalian endoribonuclease, specifically for the cleavage of RNA (deaminated RNA) between guanosine 3AE-phosphate residues (or inosine 3AE-phosphate) and the 5AE-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2AE, 3AE-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3AE-phosphate termini. This product has multiple applications in molecular cloning and DNA sequencing. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X mM. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations.
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