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Sigma Aldrich Fine Chemicals Biosciences Folin & Ciocalteu’s phenol reagent | MFCD00132625 | 100mL
Supplier: Sigma Aldrich Fine Chemicals Biosciences F9252100ML
Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration.[7][6] It has also been used for the quantification of total phenolics.[12] In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
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