Gibco™ Freedom™ ExpiCHO-S™ Kit

Catalog No.	Quantity
A46847	1 Kit

Catalog No. A46847 Supplier Gibco™ Supplier No. A46847

Description

An easy-to-use, beginning-to-end product for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. The Gibco™ Freedom™ ExpiCHO-S™ Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit.

• Seamless transition from transient to stable clones – all in one cell line
• Regulatory-friendly cGMP-banked cells
• Dual vector system provides ability to control heavy and light chain ratios
• Ability to achieve titers of 3 to 5 g/L in fed batch cultures
• Short development timeline with less than 5 months needed to get from gene of interest to clone
• Access to technical support and consultation
• Flexible, royalty-free commercial licensing options

Order Info

Kit components ship separately

Specifications

• Cell Line: ExpiCHO-S cells, cGMP Banked
• For Use With (Application): Protein Production, Stable Cell Line Development
• Quantity: 1 Kit
• Content And Storage: • Gibco ExpiCHO Expression Medium, 2–8°C, protect from light
  • ExpiCHO-S cells (cGMP-banked), liquid nitrogen
  • One Shot TOP10 Chemically Competent E. coli, –80°C
  • Freedom pCHO 3.0 Expression vectors, –5 to –20°C
  
  
• Delivery Type: Transfection
• Expression System: Mammalian

Frequently Asked Questions (FAQs)

What level of product quality is seen with the Freedom ExpiCHO-S Kit?

After execution and payment for a Commercial Production License, a Cell Line Documentation Package (>200 pages, and specific to the lot of cells purchased), is provided, which includes the entire lineage history of the cells, starting at receipt of the initial vial at Thermo Fisher Scientific through delivery to the purchaser, as well as, all testing reports.

Do you offer technical support for the Freedom ExpiCHO-S Kit?

Yes. Contact our Field Application Scientists for technical support.

How can I learn more about commercial licensing of Freedom CHO-S cells?

Please contact our licensing team at outlicensing@thermofisher.com to obtain details.

Is there a pull-through royalty on final products or an annual maintenance fee when using the Freedom ExpiCHO-S Kit?

No. There is a one-time license fee required prior to IND and an additional fee at the time of commercialization.

When is a license required for the Freedom ExpiCHO-S Kit?

A commercial use license is required prior to the sale of expressed proteins, or when an IND submission is being prepared for human clinical studies.

How many transfections are typically required to get high-expressing clones from the Freedom ExpiCHO-S Kit?

Only two transfections are recommended.

What transfection methods can be used with the Freedom ExpiCHO-S Kit?

FreeStyle MAX Reagent is included in the Freedom ExpiCHO-S Kit as the preferred transfection reagent. ExpiCHO-S Cells (cGMP banked) are also compatible with the Neon Transfection System (optimization of instrument settings is required).

What cloning methods can be used with the Freedom ExpiCHO-S Kit?

Limiting dilution cloning is recommended with the Freedom ExpiCHO-S Kit.

What selection system is used in the Freedom ExpiCHO-S Kit?

Puromycin in combination with methotrexate (MTX) is used to select for stable pools following transfection with the Freedom pCHOm 3.1 and Freedom pCHOp 3.2 vectors.

What are the main features of the Freedom pCHOm 3.1 and Freedom pCHOp 3.2 vectors?

The Freedom ExpiCHO-S two vector system provides the ability to control the ratio of heavy to light chains while allowing for consistent genomic background for transient and stable workflows.

Does the Freedom ExpiCHO-S Kit contain documentation for the ExpiCHO-S Cells (cGMP banked)?

A certificate of analysis (COA) demonstrating suitability for research use is available to everyone who purchases the Freedom ExpiCHO-S Kit or the ExpiCHO-S Cells (cGMP banked). A full documentation package, including the cell line's lineage, history, and cGMP testing results, is offered upon execution of a commercial use license.

What is included in the Freedom ExpiCHO-S Kit?

The Freedom ExpiCHO-S Kit contains all major components needed for the development of stable clones, including ExpiCHO-S Cells (cGMP banked), vectors, and media. A complete list of components, including additional materials required but not included, can be found in the ExpiCHO Expression System Kit manual (https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FMAN0025765_FreedomExpiCHO-S_Kit_UG.pdf).

Can ExpiCHO-S Cells (cGMP banked) be used for the production of commercial products?

Yes. ExpiCHO-S Cells (cGMP banked) are intended (with the purchase of a commercial license) for the development of biologics. They have been produced in a cGMP animal origin-free environment and have been scaled up in a commercial setting to produce products for clinical testing.

How successful is the Freedom ExpiCHO-S Kit in creating stable clones?

ExpiCHO-S clones producing a model IgG protein were assessed for stability by comparing volumetric productivity for over 60 generations. Four out of the five clones that were tested maintained expression levels over 60 generations. Expression levels were considered stable if the titer at the end of the stability assessment (at generation 60) was at least 70% of the titer observed at the beginning of the stability study.

How long does it take to obtain a lead clone with the Freedom ExpiCHO-S Kit?

It is possible to go from transfection to lead clone in as little as 4 months with the Freedom ExpiCHO-S Kit.

What titers can be achieved with the Freedom ExpiCHO-S Kit?

Volumetric titers of over 3 g/L in fed batch have been obtained with the Freedom ExpiCHO-S Kit.

For Research Use Only. Not for use in diagnostic procedures.