Gibco FreeStyle F17 Expression Medium
FreeStyle F17 Expression Medium
Manufacturer: Gibco A1383504
DescriptionFreeStyle F17 Expression Medium is an animal originfree, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 48 mM L-glutamine or GlutaMAX supplement is required before use. Gibco FreeStyle F17 Expression Medium features:
Little or no required adaptation
Gibco 293-F, 293-H, FreeStyle 293-F, and CHO-S cells in suspension can be cultured directly into Gibco FreeStyle F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.
Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco FreeStyle F17 Medium provides equal or better performance compared to Gibco FreeStyle 293 Expression Medium and Gibco FreeStyle CHO Medium. Gibco FreeStyle F17 Medium supports 293-F cells at a peak density of 67 x 106 viable cells per mL, and supports CHO-S cells at a peak density of 89 x 106 viable cells per mL. Cells cultured in Gibco FreeStyle F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent. Protein expression typically exceeds 75150µg/mL IgG in 293-F cells.
Animal originfree, protein-free, and chemically defined formulation
Gibco FreeStyle F17 Medium is animal originfree, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition.
Scalability in spinner flasks and bioreactors
Protein production using Gibco FreeStyle F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle F17 Medium with additional Pluronic F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco FreeStyle F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
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|ISO 13485 standard|
|FreeStyle™ F17 Expression Medium, 20L, Liquid, 1X Concentration, Serum-Free, Sterile, 2-Mercaptoethanol Supplement, For Antibody & Cell Culture Production, Mammalian Cell Culture|
|Antibody and cell culture production, mammalian cell culture|
|Qualified for further manufacturing|
|2 to 8°C|
|FreeStyle™ F17 Expression Medium|
|2 to 8°C|
Safety and Handling
ShelfLife : 12 Month(s)
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For Research Use Only. Not for use in diagnostic procedures.