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Gibco™ FreeStyle™ F17 Expression Medium Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

FreeStyle™ F17 Expression Medium

Manufacturer:  Gibco™ A1383504

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Catalog No. A1383504


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Description

Description

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco™ FreeStyle™ F17 Expression Medium features:

  • Little or no required adaptation
  • Ability to support suspension growth and transfection of 293-F and CHO cells
  • Animal origin–free, protein-free, and chemically defined formulation
  • Scalability in spinner flasks and bioreactors

    Little or no required adaptation
    Gibco™ 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco™ FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

    Ability to support suspension growth and transfection of 293-F and CHO cells
    Gibco™ FreeStyle™ F17 Medium provides equal or better performance compared to Gibco™ FreeStyle™ 293 Expression Medium and Gibco™ FreeStyle™ CHO Medium. Gibco™ FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 x 106 viable cells per mL. Cells cultured in Gibco™ FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150µg/mL IgG in 293-F cells.

    Animal origin–free, protein-free, and chemically defined formulation
    Gibco™ FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

    Scalability in spinner flasks and bioreactors
    Protein production using Gibco™ FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ F17 Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture.

    cGMP manufacturing and quality system
    Gibco™ FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Specifications

    Specifications

    ISO 13485 standard
    FreeStyle™ F17 Expression Medium, 20L, Liquid, 1X Concentration, Serum-Free, Sterile, 2-Mercaptoethanol Supplement, For Antibody & Cell Culture Production, Mammalian Cell Culture
    Antibody and cell culture production, mammalian cell culture
    Liquid
    Qualified for further manufacturing
    2 to 8°C
    Sterile
    1 x
    <1 EU/mL
    Universal bag(s)
    FreeStyle™ F17 Expression Medium
    20L
    2 to 8°C
    Safety and Handling

    Safety and Handling

    ShelfLife : 12 Month(s)

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