Invitrogen™ FT3 Competitive ELISA Kit

Catalog No.	Quantity
EEL136	96 Tests

Catalog No. EEL136 Supplier Invitrogen™ Supplier No. EEL136

Description

ELISA

The multispecies Free Triiodothyronine (fT3) ELISA quantitates fT3 in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Free Triiodothyronine (fT3) independent of species. A fT3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to fT3 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Free triiodothyronine (FT3) is a thyroid hormone that plays a critical role in regulating metabolism and energy production in the body. FT3 is produced by the thyroid gland and is responsible for increasing the metabolic rate of cells. It binds to nuclear receptors in the cell, activating transcription of genes involved in energy metabolism. FT3 also regulates the expression of genes involved in growth and development, making it essential for normal growth and development. Structurally, FT3 is a tyrosine-based hormone composed of three iodine atoms and is highly lipophilic. It circulates in the blood bound to plasma proteins and is transported to target tissues where it exerts its effects.

Specifications

• Assay Range: 1.56-100 pg/mL
• Assay Sensitivity: 0.94 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Thyroid hormone
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Free triiodothyronineFree triiodothyronine;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: FT3
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min