Invitrogen™ FT4 Competitive ELISA Kit

Catalog No.	Quantity
EEL133	96 Tests

Catalog No. EEL133 Supplier Invitrogen™ Supplier No. EEL133

Description

ELISA

The multispecies Free Thyroxine (fT4) ELISA quantitates fT4 in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Free Thyroxine (fT4) independent of species. A fT4 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to fT4 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Free Thyroxine (FT4) is a hormone produced by the thyroid gland and plays a crucial role in regulating metabolism, growth, and development in the body. It is transported in the bloodstream and acts on target cells by binding to thyroid hormone receptors. The structure of FT4 is characterized by a tyrosine residue linked to an iodine atom, forming the thyroid hormone. It has a molecular weight of 777.00 g/mol and a chemical formula of C15H11I4NO4. FT4 is primarily produced in the thyroid gland and is regulated by the hypothalamic-pituitary-thyroid axis. Its levels are typically measured in blood tests to diagnose thyroid disorders.

Specifications

• Assay Range: 1.56-100 pg/mL
• Assay Sensitivity: 0.94 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Thyroid hormone
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Free ThyroxineFree Thyroxine;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: FT4
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min