Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells
Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
Supplier: Invitrogen™ T1027
- Homogeneous assay format allows detection of β-galactosidase in presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
- The kinetics of glow-assay provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
- Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
- Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
- Assay sensitivity is 100 to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
- Assay Type: β-Gal (lacZ) Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Galacton-Star™
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
- Technique: Enhanced Chemiluminescence
|β-Galactosidase Reporter Gene Assay System|
|This kit contains the enzyme substrate and the reaction buffer.
Store the product at 4°C.
|β-Gal (lacZ) Assay|
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For Research Use Only. Not for use in diagnostic procedures.