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Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells Non-distribution product as customer accommodation.

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Manufacturer:  Invitrogen™ T1030

Catalog No. T1030



Includes: Enzyme substrate and reaction buffer

Description

Description

Gal-Screen homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.
  • Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
  • The kinetics of the glow-assay provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
  • Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
  • Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
  • Assay sensitivity is 100 to 1000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
  • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
  • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
  • Assay can be completed in about one hour, providing fast assay turnaround
Specifications
  • Compatible Cells: Mammalian Cells, Yeast Cells
  • Detection Method: Chemiluminescent
  • For Use With (Equipment): Luminometer (Microplate)
  • Form: Liquid
  • Format: 96-well plate
  • High Throughput Compatibility: High Throughput-Compatible
  • Readout: End Point
  • Substrate: Galacton-Star
  • Substrate Properties: Chemical Substrate
  • Substrate Type: Beta-Gal Substrate
  • Target Enzyme: Beta-Galactosidase
Specifications

Specifications

Reporter Gene Assays
96-well plate
Detection of β-galactosidase in mammalian cells
1000 assays
Enhanced Chemiluminescence
Enzyme substrate and reaction buffer
Luminometer
Store at 2° to 8°C
Documents
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