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Invitrogen™ Galectin-3 (LGALS3) Rat ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ ERLGALS3

Encompass

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Catalog No. ERLGALS3


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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Rat Galectin-3 (LGALS3) ELISA quantitates Rt Galectin-3 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Galectin-3. Principle of the method The Rat Galectin-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
Specifications

Specifications

Colorimetric Microplate Reader
ELISA Kit
Biotin
TMB
HRP
3.28-800 pg/ml
Galectin-3 (LGALS3) ELISA Kit, 96 Assays, 1 Kit, Rat Species, Chemokines and Receptors Protein Family, Immunology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Galectin-3 (Lgals3)
83781
RUO
2°C to 8°C
4 hr. 45 min.
Rat and mouse galectin-3
Immunology
<12%
96 Tests
Plasma, Serum, Supernatant
AGE-R3,CBP30,L-34,gal-3
96 Assays
This ELISA pair antibody detects rat and mouse Galectin-3.
Chemokine & Receptors
Solid-phase sandwich ELISA
P08699
3pg/mL to 800pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Rat galectin-3 precoated 96-well strip plate, lyophilized recombinant rat galectin-3 standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X conc
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Rat
Rat
AGE-R3, CBP30, L-34, gal-3
<10%
Rat
3 pg/ml
Colorimetric
SDS
Documents
Product Certifications
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