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Invitrogen™ Gateway™ pENTR™ 11 Dual Selection Vector Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Gateway™ pENTR™ 11 Dual Selection Vector

Manufacturer:  Invitrogen™ A10467

Catalog No. A10467


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Description

Description

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:
  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    o negative selection and
    o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli

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  • Specifications

    Specifications

    Gateway™ pENTR™ 11 Dual Selection Vector, 10μg, Kozak Sequence for Efficient Initiation of Translation in Eukaryotic Cells, Two E. coli Ribosome Binding Sites for Efficient Initiation of Translation in Prokaryotic Cells, Designed to Clone DNA Sequences
    20μL of pENTR™ dual selection vector at 0.5μg/μL, in TE buffer, pH 8.0 (10mM Tris-HCl, 1mM EDTA, pH 8.0)
    Gateway™ pENTR™ 11 Dual Selection Vector
    –20°C
    Untagged
    Cloning, entry vectors and kits, Gateway cloning, Kozak sequence for efficient initiation of translation in eukaryotic cells. Two E. coli ribosome binding sites for efficient initiation of translation in prokaryotic cells
    8
    10μg
    TE buffer
    Safety and Handling

    Safety and Handling

    ShelfLife : 6 Month(s)

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    For Research Use Only. Not for use in diagnostic procedures.