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Invitrogen™ Gateway™ pENTR™ 3C Dual Selection Vector

Catalog No. A10464
Encompass_Preferred
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Quantity:
10 μg

Gateway™ pENTR™ 3C Dual Selection Vector

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:

  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    • o negative selection and
    • o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli
TRUSTED_SUSTAINABILITY

Specifications

Product Type Dual SelectionExpression Vector
Content And Storage 10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.
Store at -20°C
Antibiotic Resistance Bacterial Chloramphenicol (CmR), Kanamycin (KanR)
Cleavage No Cleavage Site
Protein Tag Untagged
Cloning Method Gateway
Quantity 10 μg
Vector pENTR
Product Line pENTR™

For Research Use Only. Not for use in diagnostic procedures.

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