Invitrogen™ Gateway™ pENTR™ 3C Dual Selection Vector  

Gateway™ pENTR™ 3C Dual Selection Vector

Manufacturer:  Invitrogen™ A10464

Catalog No. A10464

Description

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:

attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression

Kozak consensus sequence for efficient translation initiation in eukaryotic systems

Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)

rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli

pUC origin for high-copy replication and maintenance of the plasmid in E. coli

Kanamycin resistance gene for selection in E. coli

The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
o negative selection and
o Chloramphenicol selection in E. coli

Kanamycin resistance gene for selection in E. coli

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Specifications

• Description: Gateway™ pENTR™ 3C Dual Selection Vector, 10μg, Three Reading Frames, Kozak Sequence for Efficient Initiation of Translation in Eukaryotic Cells, E. coli Ribosome Binding Site for Efficient Initiation, Entry Vectors are Designed to Clone DNA
• Includes: 20μL of pENTR™ dual selection vector at 0.5μg/μL, in TE buffer, pH 8.0 (10mM Tris-HCl, 1mM EDTA, pH 8.0)
• Product Type: Gateway™ pENTR™ 3C Dual Selection Vector
• Storage Requirements: –20°C
• Protein Tag: Untagged

• For Use With (Application): Three reading frames available, Kozak sequence for efficient initiation of translation in eukaryotic cells, E. coli ribosome binding site for efficient initiation, cloning, entry vectors and kits, Gateway cloning
• pH: 8
• Quantity: 10μg
• Storage Buffer: TE buffer

Safety and Handling

ShelfLife : 6 Month(s)

For Research Use Only. Not for use in diagnostic procedures.