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Sigma Aldrich Fine Chemicals Biosciences GC-RICH PCR System

Catalog No. 502906138
Encompass

The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase a thermostable enzyme with a proofreading (3-5 exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH reSOLUTIONon SOLUTIONon allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 mul PCR.ContentsGC-RICH Enzyme Mix in storage bufferGC-RICH Reaction Buffer 5x concentrated with 7.5 mM MgCl2 and DMSOGC-RICH ReSOLUTIONon SOLUTIONon 5 MMgCl2 Stock SOLUTIONon 25 mMWater PCR Grade

Catalog No. 50-290-6138 Supplier Sigma Aldrich Fine Chemicals Biosciences Supplier No. 12140306001
May include imposed supplier surcharges.
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The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase a thermostable enzyme with a proofreading (3-5 exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH reSOLUTIONon SOLUTIONon allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 mul PCR.ContentsGC-RICH Enzyme Mix in storage bufferGC-RICH Reaction Buffer 5x concentrated with 7.5 mM MgCl2 and DMSOGC-RICH ReSOLUTIONon SOLUTIONon 5 MMgCl2 Stock SOLUTIONon 25 mMWater PCR Grade

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