Invitrogen™ Human GDF15 ELISA Kit

Catalog No.	Quantity
BMS2258	96 Tests

Catalog No. BMS2258 Supplier Invitrogen™ Supplier No. BMS2258

Description

ELISA

The Human GDF15 ELISA quantitates Hu GDF15 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GDF15. Principle of the method The Human GDF15 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

GDF-15 belongs to the TGF-beta cytokine family, whose members play an important role during prenatal development and postnatal growth, and the remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in the placenta and, to a much lesser extent, in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest GDF-15's involvement in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.

Specifications

• Accession Number: Q99988
• Assay Range: 2-125 pg/mL
• Assay Sensitivity: 0.59 pg/mL
• Content And Storage: • Aluminum pouch(es) with a microplate coated with monoclonal antibody to human GDF15
  • Biotin-conjugate anti-human GDF15 polyclonal antibody
  • Streptavidin-HRP
  • Human GDF15 standard, lyophilized, 250 pg/mL upon reconstitution
  • Sample diluent
  • Assay buffer concentrate, 20X (PBS with 1% Tween 20 and 10% BSA)
  • Wash buffer concentrate, 20X (PBS with 1% Tween 20)
  • Substrate solution (tetramethyl-benzidine)
  • Stop solution (1 M phosphoric acid)
  • Adhesive films
  
  
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Gdf15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; macrophage inhibiting cytokine-1; macrophage inhibitory cytokine 1; MIC1; MIC-1; NAG1; NAG-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; placental TGF-beta; Prostate differentiation factor; PTGFB; PTGF-beta; SBFGdf15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; macrophage inhibiting cytokine-1; macrophage inhibitory cytokine 1; MIC1; MIC-1; NAG1; NAG-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; placental TGF-beta; Prostate differentiation factor; PTGFB; PTGF-beta; SBF;
• Gene ID (Entrez): 9518
• Gene Symbol: Gdf15
• Interassay CV: 2.9%
• Intraassay CV: 4.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: GDF15
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov