Gelacell - PCL:PLGA scaffolds fixed to cell crowns in 12-well plate for 3D cell culture (1 plate). INTRO: Gelacell, manufactured by Gelatex Technologies, is a unique, 3D microfibrous scaffold specifically engineered for advanced in vitro 3D cell culture and tissue engineering applications. DESCRIPTION: This variant of Gelacell is specially designed for medium-to-long term cell culture durations. Scaffolds made from pharmaceutical grade polycaprolactone + poly lactic-co-glycolic acid (PCL:PLGA ratio is 70:30) are fixed to cell crowns in a 12-well plate. The cell crowns act as Boyden chambers for facilitating studies related to air-liquid interface culture, co-culture, complex tissue models, etc. The optimized design ensures thermal, chemical, and mechanical stability, while also providing substantial swelling capabilities and porosity, allowing efficient nutrient diffusion and thereby preventing cellular waste build up.