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Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology Non-distribution product as customer accommodation.

A ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System

Manufacturer:  Invitrogen™ A13287

Catalog No. A13287


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Description

Description

GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™.

Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
  • TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
  • ARS⁄CEN origin to allow stable maintenance of the construct in yeast
  • F’ for conjugation
  • oriT for maintenance of large constructs in E.coli
  • Spectinomycin resistance for selection in E.coli
  • 6 Convenient and validated restriction sites flanking the cloning region
For Research Use Only. Not for use in diagnostic procedures.
Specifications

Specifications

GeneArt™ pYES1L Vector with Sapphire™ Technology, 10 rxns, Seamless Cloning Method, Spectinomycin (Bacterial) Antibiotic Resistance, Ready-to-Use, 9.3kb Linearized BAC/YAC Shuttle Vector, Used for the Assembly of DNA Fragments
The product contains 5 vials of linear GeneArt™ pYES1L Vector with Sapphire Technology™ at a concentration of 50ng⁄mL. Each vial contains enough plasmid for 2 cloning reactions using the GeneArt™ High-Order Genetic Assembly System (needs to be purchased separately).
10 reactions
GeneArt™ pYES1L Vector with Sapphire™ Technologyy
110 kb total (vector plus all inserts)
Stable
Seamless Cloning Vector, Linear
PCR cloning, seamless cloning and genetic assembly
8
Up to 10 Fragments
TRP1
10 rxn(s)
Store the product at -80°C. The vector is guaranteed stable for 6 months when properly stored.
Safety and Handling

Safety and Handling

ShelfLife : 6 Month(s)

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