Thermo Scientific™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

Description

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line.

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER beta-UAS-bla GripTite™293 cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

Specifications

• Cell State: Dividing Cells
• Content And Storage: ER beta-UAS-bla GriptTite™ cells (K1091)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Druggable Target: Nuclear Receptors
• No. of Cells: 2 x 10⁶ Cells
• Product Line: GeneBLAzer™
• Product Type: GripTite Cells
• Quantity: 2 x 10⁶ cells
• Reporter Gene: BLA (Beta-Lactamase)
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.