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Thermo Scientific™ GeneBLAzer™ ERRα-UAS-bla HEK 293T Cells

Catalog No. K1702
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K1702 2 x 106 cells
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Catalog No. K1702 Supplier Thermo Scientific™ Supplier No. K1702
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Description

ERR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Estrogen related receptor alpha (ERR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer UAS-bla HEK 293T cell line.

ERR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Estrogen related receptor alpha (ERR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of XCT790, an inverse agonist. In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists L165, 041, GW 1929, GW 7647, GW 0742
Antagonists T0070907, GW 9662, GW 6471
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage GeneBLAzer™ Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
Druggable Target Nuclear Receptors
No. of Cells 2 x 106 Cells
Primary Agonist Rosiglitazone
Product Line GeneBLAzer
Product Type HEK 293T Cells
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry ESRRA, NR3B1, ERR1, ERR alpha
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For Research Use Only. Not for use in diagnostic procedures.

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